Avezov LabCecile Crapart, Zubenelgenubi C. Scott,2Tasuku Konno, Aman Sharma, David M. D. Bailey, Edward Avezov* and Elena F. Koslover* (2023) Luminal transport rates through intact endoplasmic reticulum limit the magnitude of localized Ca2+signals. BioRxiv 2023.06.23.546357 https://doi.org/10.1101/2023.06.23.546357
Mukadam, A. S., Miller, L. V. C., Smith, A. E., Vaysburd, M., Sakya, S. A., Sanford, S., Keeling, S., Tuck, B. J., Katsinelos, T., Green, C., Skov, L., Kaalund, S. S., Foss, S., Mayes, K., O’Connell, K., Wing, M., Knox, C., Banbury, J., Avezov, E., … McEwan, W. A. (2023). Cytosolic antibody receptor TRIM21 is required for effective tau immunotherapy in mouse models. Science (New York, N.Y.), 379(6639), 1336–1341. https://doi.org/10.1126/SCIENCE.ABN1366
Lu, M., Christensen, C. N., Weber, J. M., Konno, T., Läubli, N. F., Scherer, K. M., Avezov, E., Lio, P., Lapkin, A. A., Kaminski Schierle, G. S., & Kaminski, C. F. (2023). ERnet: a tool for the semantic segmentation and quantitative analysis of endoplasmic reticulum topology. Nature Methods 2023 20:4, 20(4), 569–579. https://doi.org/10.1038/s41592-023-01815-0
PH Htet, E Avezov, E Lauga, Fluid mechanics of luminal transport in actively contracting endoplasmic reticulum, bioRxiv, 2023.01. 11.523552
Parutto, P., Heck, J., Lu, M., Kaminski, C., Avezov, E., Heine, M., and Holcman, D. (2022). High-throughput super-resolution single-particle trajectory analysis reconstructs organelle dynamics and membrane reorganization. Cell Reports Methods 2, 100277. https://doi.org/10.1016/J.CRMETH.2022.100277.
Chyi Wei Chung, Amberley D. Stephens, Tasuku Konno, Edward Ward, Edward Avezov, Clemens F. Kaminski, Ali A. Hassanali, and Gabriele S. Kaminski Schierle, Intracellular Aβ42 Aggregation Leads to Cellular Thermogenesis, Journal of the American Chemical Society (2022) 144 (22), 10034-10041DOI: 10.1021/jacs.2c03599
E. P. Melo, T. Konno, I. Farace, M. A. Awadelkareem, L. R. Skov, F. Teodoro, T. P. Sancho, A. W. Paton, J. C. Paton, M. Fares, P. M. R. Paulo, X. Zhang, E. Avezov, Stress-induced protein disaggregation in the endoplasmic reticulum catalysed by BiP. Nature Communications. 13, 2501 (2022). doi: 10.1038/s41467-022-30238-2
J. E. Chambers, N. Zubkov, M. Kubánková, J. Nixon-Abell, I. Mela, S. Abreu, M. Schwiening, G. Lavarda, I. López-Duarte, J. A. Dickens, T. Torres, C. F. Kaminski, L. J. Holt, E. Avezov, J. A. Huntington, P. S. George-Hyslop, M. K. Kuimova, S. J. Marciniak, Z-α1-antitrypsin polymers impose molecular filtration in the endoplasmic reticulum after undergoing phase transition to a solid state. Science Advances. 8, eabm2094 (2022). DOI: 10.1126/sciadv.abm2094
Arnaud Blomme, Coralie Peter et al. THEM6-mediated lipid remodelling sustains stress resistance in cancer, EMBO Molecular Medicine (2022)e14764 https://doi.org/10.15252/emmm.202114764
Tasuku Konno, Pierre Parutto, David M. D. Bailey, Valentina Davì, Cécile Crapart, Mosab Ali Awadelkareem, Colin Hockings, Aidan Brown, Katherine M. Xiang, AnamikaAgrawal, Joseph E. Chambers, Molly Vander Werp, Katherine Koning, EmmanouilMetzakopian, Laura Westrate, Elena Koslover, Edward Avezov: Endoplasmic Reticulum morphological regulation by RTN4/NOGO modulates neuronal regeneration by curbing luminal transport, bioRxiv 2021.05.10.441946; doi: https://doi.org/10.1101/2021.05.10.441946
Eduardo Pinho Melo, Ilaria Farace, Tasuku Konno, Mosab Ali Awadelkareem, Lise R.Skov, Teresa P. Sancho, Adrienne W. Paton, James C. Paton, Matthew Fares, Pedro M. R. Paulo, Xin Zang, Edward Avezov: Stress-induced protein disaggregation in the Endoplasmic Reticulum catalysed by BiP, bioRxiv 2021.05.09.442953; doi: https://doi.org/10.1101/2021.05.09.442953
THEM6-mediated lipid remodelling sustains stress resistance in cancerArnaud Blomme, Coralie Peter, Ernest Mui, Giovanny Rodriguez Blanco, Louise M.Mason, Lauren E. Jamieson, Grace H. McGregor, Sergio Lilla, Chara Ntala, RachanaPatel, Marc Thiry, Sonia H. Y. Kung, Catriona A. Ford, Linda K. Rushworth, David J.McGarry, Susan Mason, Peter Repiscak, Colin Nixon, Mark J. Salji, Elke Markert, Gillian M. MacKay, Jurre J. Kamphorst, Duncan Graham, Karen Faulds, Ladan Fazli, Martin E. Gleave, Edward Avezov, Joanne Edwards, Huabing Yin, David Sumpton, Karen Blyth, Pierre Close, Daniel J. Murphy, Sara Zanivan, Hing Y. Leung bioRxiv 2021.04.04.438378; doi: https://doi.org/10.1101/2021.04.04.438378
Konno, Tasuku; Melo, Eduardo P.; Chambers, Joseph E.; Avezov, Edward: Intracellular Sources of ROS/H2O2 in Health and Neurodegeneration: Spotlight on Endoplasmic Reticulum, 25 Jan 2021 Cells 10, no. 2: 233. https://doi.org/10.3390/cells10020233
Meng Lu, Francesca W. van Tartwijk, Julie Qiaojin Lin, Wilco Nijenhuis, Pierre Parutto, Marcus Fantham, Charles N. Christensen, Edward Avezov, Christine E. Holt, Alan Tunnacliffe, David Holcman, Lukas Kapitein, Gabriele S. Kaminski Schierle, and Clemens F. Kaminski: The structure and global distribution of the endoplasmic reticulum network are actively regulated by lysosomes, Science Advances, 16 Dec 2020: Vol. 6, no. 51, eabc7209, DOI: 10.1126/sciadv.abc7209
Holcman, David and Parutto, Pierre and Chambers, Joseph E. and Fantham, Marcus and Young, Laurence J. and Marciniak, Stefan J. and Kaminski, Clemens F. and Ron, David and Avezov, Edward: Single particle trajectories reveal active endoplasmic reticulum luminal flow , Nature Cell Biology, 2018., DOI:10.1038/s41556-018-0192-2
Joseph E Chambers, Marketa Kubankova, Roland G Huber, Ismeal López-Duarte, Edward Avezov, Peter J Bond, Stefan J Marciniak, Marina K. Kuimova: An Optical Technique for Mapping Microviscosity Dynamics in Cellular Organelles. ACS Nano 04/2018; 12(5)., DOI:10.1021/acsnano.8b00177
Eduardo Pinho Melo, Carlos Lopes, Peter Gollwitzer, Stephan Lortz, Sigurd Lenzen, Ilir Mehmeti, Clemens F. Kaminski, David Ron, Edward Avezov: TriPer, an optical probe tuned to the endoplasmic reticulum tracks changes in luminal H2O2. BMC Biology 12/2017; 15(1)., DOI:10.1186/s12915-017-0367-5
Ilir Mehmeti, Stephan Lortz, Edward Avezov, Anne Jörns, Sigurd Lenzen: ER- resident antioxidative GPx7 and GPx8 enzyme isoforms protect insulin-secreting INS-1E β-cells against lipotoxicity by improving the ER antioxidative capacity. Free Radical Biology and Medicine 07/2017; 112., DOI:10.1016/j.freeradbiomed.2017.07.021
Thomas S. Blacker, WeiYue Chen, Edward Avezov, Richard J Marsh, Michael R. Duchen, Clemens F. Kaminski, Angus J Bain: Investigating State Restriction in Fluorescent Protein FRET Using Time-Resolved Fluorescence and Anisotropy. The Journal of Physical Chemistry C 12/2016; 121(3)., DOI:10.1021/acs.jpcc.6b11235
K Kollmann, W Warsch, C Gonzalez-Arias, F L Nice, E Avezov, J Milburn, J Li, D Dimitropoulou, S Biddie, M Wang, E Poynton, M Colzani, M R Tijssen, S Anand, U McDermott, B Huntly, T Green: A novel signalling screen demonstrates that CALR mutations activate essential MAPK signalling and facilitate megakaryocyte differentiation. Leukemia 10/2016; 31(4)., DOI:10.1038/leu.2016.280
Navit Ogen-Shtern, Edward Avezov, Marina Shenkman, Ron Benyair, Gerardo Z Lederkremer: Mannosidase IA is in Quality Control Vesicles and Participates in Glycoprotein Targeting to ERAD. Journal of Molecular Biology 04/2016; 428(16)., DOI:10.1016/j.jmb.2016.04.020
Tasuku Konno, Eduardo Pinho Melo, Carlos Lopes, Ilir Mehmeti, Sigurd Lenzen, David Ron, Edward Avezov: ERO1-independent production of H2O2 within the endoplasmic reticulum fuels Prdx4-mediated oxidative protein folding. The Journal of Cell Biology 10/2015; 211(2):253-259., DOI:10.1083/jcb.201506123
Steffen Preissler, Joseph E. Chambers, Ana Crespillo-Casado, Edward Avezov, Elena Miranda, Juan Perez, Linda M. Hendershot, Heather P. Harding, David Ron: Physiological modulation of BiP activity by trans-protomer engagement of the interdomain linker. eLife Sciences 10/2015
WeiYue Chen, Edward Avezov, Simon C Schlachter, Fabrice Gielen, Romain F Laine, Heather P Harding, Florian Hollfelder, David Ron, Clemens F Kaminski: A Method to Quantify FRET Stoichiometry with Phasor Plot Analysis and Acceptor Lifetime Ingrowth. Biophysical Journal 03/2015; 108(5):999-1002., DOI:10.1016/j.bpj.2015.01.012
Edward Avezov, Tasuku Konno, Alisa Zyryanova, Weiyue Chen, Romain Laine, Ana Crespillo-Casado, Eduardo Pinho Melo, Ryo Ushioda, Kazuhiro Nagata, Clemens F Kaminski, Heather P Harding, David Ron: Retarded PDI diffusion and a reductive shift in poise of the calcium depleted endoplasmic reticulum. BMC Biology 01/2015; 13(1):2., DOI:10.1186/s12915-014-0112-2
Pawan Gulati, Edward Avezov, Marcella Ma, Robin Antrobus, Paul J Lehner, Stephen O’Rahilly, Giles S H Yeo: Fat mass and obesity-related (FTO) shuttles between the nucleus and cytoplasm. Bioscience Reports 09/2014; 34(5)., DOI:10.1042/BSR20140111
Satoshi Tsunoda, Edward Avezov, Alisa Zyryanova, Tasuku Konno, Leonardo Mendes-Silva, Eduardo Pinho Melo, Heather P Harding, David Ron: Intact protein folding in the glutathione-depleted endoplasmic reticulum implicates alternative protein thiol reductants. eLife Sciences 07/2014;, DOI:10.7554/eLife.03421
Jyoti Nangalia, Charles E Massie, E Joanna Baxter, Francesca L Nice, Gunes Gundem, David C Wedge, Edward Avezov, Juan Li, Karoline Kollmann, David G Kent, Athar Aziz, Anna L Godfrey, Jonathan Hinton, Inigo Martincorena, Peter Van Loo, Amy V Jones, Paola Guglielmelli, Patrick Tarpey, Heather P Harding, John D Fitzpatrick, Calum T Goudie, Christina A Ortmann, Stephen J Loughran, Keiran Raine, David R Jones, Adam P Butler, Jon W Teague, Sarah O’Meara, Stuart McLaren, Michele Bianchi, Yvonne Silber, Danai Dimitropoulou, David Bloxham, Laura Mudie, Mark Maddison, Ben Robinson, Clodagh Keohane, Cathy Maclean, Kate Hill, Kim Orchard, Sudhir Tauro, Ming-Qing Du, Mel Greaves, David Bowen, Brian J P Huntly, Claire N Harrison, Nicholas C P Cross, David Ron, Alessandro M Vannucchi, Elli Papaemmanuil, Peter J Campbell, Anthony R Green: Somatic CALR Mutations in Myeloproliferative Neoplasms with Nonmutated JAK2. New England Journal of Medicine 12/2013; 369(25)., DOI:10.1056/NEJMoa1312542
Edward Avezov, Benedict C S Cross, Gabriele S Kaminski Schierle, Mikael Winters, Heather P Harding, Eduardo Pinho Melo, Clemens F Kaminski, David Ron: Lifetime Imaging of a fluorescent protein sensor reveals surprising stability of ER thiol redox. The Journal of Cell Biology 04/2013; 201(2):337-49., DOI:10.1083/jcb.201211155
Marina Shenkman, Bella Groisman, Efrat Ron, Edward Avezov, Linda M Hendershot, Gerardo Z Lederkremer: A Shared Endoplasmic Reticulum-associated Degradation Pathway Involving the EDEM1 Protein for Glycosylated and Nonglycosylated Proteins. Journal of Biological Chemistry 12/2012; 288(4)., DOI:10.1074/jbc.M112.438275
Gali Soria, Yaeli Lebel-Haziv, Marcelo Ehrlich, Tsipi Meshel, Adva Suez, Edward Avezov, Perri Rozenberg, Adit Ben-Baruch: Mechanisms Regulating the Secretion of the Promalignancy Chemokine CCL5 by Breast Tumor Cells: CCL5’s 40s Loop and Intracellular Glycosaminoglycans 1 2. Neoplasia (New York, N.Y.) 01/2012; 14(1):1-19., DOI:10.1593/neo.111122
Edward Avezov, Efrat Ron, Yana Izenshtein, Yosef Adan, Gerardo Z Lederkremer: Pulse-chase Analysis of N-linked Sugar Chains from Glycoproteins in Mammalian Cells. Journal of Visualized Experiments 04/2010; 38(38):1899., DOI:10.3791/1899
Edward Avezov, Zehavit Frenkel, Marcelo Ehrlich, Annette Herscovics, Gerardo Z Lederkremer: Endoplasmic Reticulum (ER) Mannosidase I Is Compartmentalized and Required for N-Glycan Trimming to Man5–6GlcNAc2 in Glycoprotein ER-associated Degradation. Molecular biology of the cell 02/2008; 19(1):216-25., DOI:10.1091/mbc.E07-05-0505
Maria Kondratyev, Edward Avezov, Marina Shenkman, Bella Groisman, Gerardo Z Lederkremer: PERK-dependent compartmentalization of ERAD and unfolded protein response machineries during ER stress. Experimental Cell Research 11/2007; 313(16):3395-407., DOI:10.1016/j.yexcr.2007.07.006
Bella Groisman, Edward Avezov, Gerardo Z. Lederkremer: The E3 Ubiquitin Ligases HRD1 and SCFFbs2 Recognize the Protein Moiety and Sugar Chains, Respectively, of an ER-Associated Degradation Substrate. Israel Journal of Chemistry 03/2006; 46(2):189-196., DOI:10.1560/2QPD-9WP9-NCYK-58X3
